Uricase and its clinical applications
نویسندگان
چکیده
More than three decades ago allopurinol was considered as a first line drug which regulate uric acid synthesis by inhibiting xanthine oxidase (XO) enzyme. . In addition to allopurinol, febuxostat is also used to reduce the synthesis of uric acid by inhibiting the XO enzyme and is approved by the European Medical Agency in 2008 and US FDA in 2009. . In contrast to allopurinol it acts on both reduced as well as oxidised form of XO. . Partial relief from inflammation can be obtained by administration of hydroxychloroquine and non steroidal anti-inflammatory drugs. .
منابع مشابه
مهندسی آنزیم درمانی اوریکاز آسپرژیلوس فلاووس با استفاده از روش جهشزایی هدفدار
Background & Aims: As a therapeutic enzyme, Aspergillus flavus (uricase or; EC 1.7.3.3), is used for treatment of urate deposits, gout and nephropathy, hyperuricemia and tumor lysis syndrom (TLS). Despite desirable kinetic features, fragile stability of uricase limits its wide range applications. Therefore, several approaches have developed such as protein engineering and genetic manipulations ...
متن کاملکلونینگ و بیان آنزیم درمانی اوریکاز آسپرژیلوس فلاووس در اشریشیاکلی
Background and Objective: Uricase (EC 1.7.3.3) is an enzyme which catalyses uric acid to H2O2 and alantoin and is utilized in the treatment of hyperuricemia or gout. The aim of this study was cloning of Aspergillus flavus uricase synthetic gene into pET-28(a) and its recombinant expression in E. coli Materials and Methods: The coding sequence of uricase retrieved from gene bank and once opti...
متن کاملA new practical system for evaluating the pharmacological properties of uricase as a potential drug for hyperuricemia.
The use of uricase-deficient mammals to screen formulations of engineered uricases as potential drugs for hyperuricemia involves heavy costs and presents a technical bottleneck. Herein, a new practical system was investigated to evaluate the pharmacological significance of a bacterial uricase based on its ability to eliminate uric acid in plasma in vitro, its pharmacokinetics in vivo in healthy...
متن کاملComparison of four methods for measuring uric acid: copper-chelate, phosphotungstate, manual uricase, and automated kinetic uricase.
Glutathione,ascorbicacidand many commonly used antibiotics falsely elevate uric acid concentrations when measured with the Du Pont “aca,” in which the copper-chelate method is used. L-Dopa interferes with both the copper-chelate method and the Technicon “SMA 12/60” phosphotungstate method. A new kinetic uricase method has been developed for use on the Du Pont aca. This kinetic uricase method ha...
متن کاملCharacterization of the common bean uricase II and its expression in organs other than nodules.
Uricase II is a purine metabolic enzyme highly induced in root nodules during the symbiosis established between legumes and bacteria of the genera Rhizobium and Bradyrhizobium. Here we describe the characterization of bean (Phaseolus vulgaris) nodule uricase II cDNA and show that uricase II is encoded by a single gene in the bean genome. This gene is also expressed in cotyledons, roots, and hyp...
متن کاملComparison of carbonate and uricase-carbonate methods for the determination of uric acid in serum.
A colorimetric carbonate procedure for the determination of uric acid has been modified to include incubation of serum with uricase to destroy uric acid. Residual nonurate chromogens are subtracted from total chromogens to obtain the concentration of “true” uric acid. Result obtained by the carbonate and the uricase-carbonate methods were in good agreement. The mean concentration of nonurate ch...
متن کامل